DBNascent | Samples

SRR1806580 Sample ID 119

**Data sources**
Paper identifier	Andrade2015dna
SRP accession	SRP055100
GEO accession	GSE65985
Samples	SRR1806580

**Technical details**
QC score	3
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	60 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	365 Kb
dREG bidirectional regions	BED file	165 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	skin fibroblast
Clone/Individual	GM00739
Strain
Genotype	Csb CS1AN mut
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-30 min	0 min	30 min
treatment	ultraviolet C (1 J/m^2/s)	-7210 s	-7200 s	10 s

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq; Cockayne syndrome fibroblasts (GM00739)
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	52
Read depth after trimming	33,513,615
Duplication proportion	0.73429
Proportion reads mapped	0.9735
Exon/intron ratio	3.2175
Unique read proportion	0.31852

**Bidirectionals**
Total # Tfit bidirectionals	4188
# Promoter Tfit bidirectionals	897
# Intronic Tfit bidirectionals	2112
# Intergenic Tfit bidirectionals	352
Total # dREG bidirectionals	3806
# Promoter dREG bidirectionals	1155
# Intronic dREG bidirectionals	1808
# Intergenic dREG bidirectionals	497
Part of Tfit master merge?	True
Part of dREG master merge?	True