DBNascent | Samples

SRR1916552 Sample ID 661

**Data sources**
Paper identifier	Elkon2015myc
SRP accession	SRP056201
GEO accession	GSE66928
Samples	SRR1916552

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	165 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	U2OS
Clone/Individual
Strain
Genotype	dox-inducible Myc (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	water	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Poor methods: assuming "mock treatment" means water and that the construct is stably integrated, but neither explicitly stated
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	111,751,547
Duplication proportion	0.77515
Proportion reads mapped	0.9553
Exon/intron ratio	1.65887
Unique read proportion	0.36975

**Bidirectionals**
Total # Tfit bidirectionals	43271
# Promoter Tfit bidirectionals	13276
# Intronic Tfit bidirectionals	18058
# Intergenic Tfit bidirectionals	10905
Total # dREG bidirectionals	30443
# Promoter dREG bidirectionals	8610
# Intronic dREG bidirectionals	11878
# Intergenic dREG bidirectionals	9461
Part of Tfit master merge?	True
Part of dREG master merge?	True