SRR1930087 Sample ID 2342

Data sources
Paper identifier Stender2017structural
SRP accession SRP056552
GEO accession GSE67295
Samples SRR1930087
Technical details
QC score 3
Replicate 2
Protocol GRO-seq
Library prep circularization
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 37 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 680 Kb
dREG bidirectional regions BED file 143 Kb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type MCF7
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment 17-beta-estradiol -30 min 0 min 30 min
Extended notes
Pipeline info and software versions
Sample notes not clear on whether vehicle is DMSO or ethanol for this experiment, doesn't explicitly say treatment concentrations for GRO-seq exps
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 51
Read depth after trimming 11,339,089
Duplication proportion 0.49253
Proportion reads mapped 0.7372
Exon/intron ratio 3.84252
Unique read proportion 0.49307
Bidirectionals
Total # Tfit bidirectionals 7754
# Promoter Tfit bidirectionals 3175
# Intronic Tfit bidirectionals 3215
# Intergenic Tfit bidirectionals 778
Total # dREG bidirectionals 3312
# Promoter dREG bidirectionals 1808
# Intronic dREG bidirectionals 976
# Intergenic dREG bidirectionals 491
Part of Tfit master merge? True
Part of dREG master merge? True