DBNascent | Samples

SRR1930090 Sample ID 2344

**Data sources**
Paper identifier	Stender2017structural
SRP	SRP056552
Samples	SRR1930090

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	35 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	709 Kb
dREG bidirectional regions	BED file	177 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	not clear on whether vehicle is DMSO or ethanol for this experiment, doesn't explicitly say treatment concentrations for GRO-seq exps
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	13,658,960
Duplication proportion	0.64145
Proportion reads mapped	0.798
Exon/intron ratio	4.18908
Unique read proportion	0.36268

**Bidirectionals**
Total # Tfit bidirectionals	8102
# Promoter Tfit bidirectionals	3978
# Intronic Tfit bidirectionals	2879
# Intergenic Tfit bidirectionals	713
Total # dREG bidirectionals	4115
# Promoter dREG bidirectionals	2620
# Intronic dREG bidirectionals	1028
# Intergenic dREG bidirectionals	492
Part of Tfit master merge?	True
Part of dREG master merge?	True