DBNascent | Samples

SRR1991267 Sample ID 2300

**Data sources**
Paper identifier	Sigova2015transcription
SRP accession	SRP057598
GEO accession	GSE68197
Samples	SRR1991267

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	14 Mb
Stranded gene read counts	TXT file	895 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	40 Kb
dREG bidirectional regions	BED file	21 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual
Strain
Genotype	BirA knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Major poly-A contamination; Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	40
Read depth after trimming	16,918,036
Duplication proportion	0.85788
Proportion reads mapped	0.4024
Exon/intron ratio	0.99608
Unique read proportion	0.12429

**Bidirectionals**
Total # Tfit bidirectionals	469
# Promoter Tfit bidirectionals	181
# Intronic Tfit bidirectionals	189
# Intergenic Tfit bidirectionals	85
Total # dREG bidirectionals	498
# Promoter dREG bidirectionals	126
# Intronic dREG bidirectionals	120
# Intergenic dREG bidirectionals	232
Part of Tfit master merge?	True
Part of dREG master merge?	True