DBNascent | Samples

SRR1991268 Sample ID 2300

**Data sources**
Paper identifier	Sigova2015transcription
SRP	SRP057598
Samples	SRR1991268

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	6 Mb
Stranded gene read counts	TXT file	882 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	5 Kb
dREG bidirectional regions	BED file	6 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual
Strain
Genotype	BirA knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	actinomycin D (1 microM)	-6 hr	0 hr	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Sparse due to major poly-A contamination and high duplication; Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	2
Raw read length	40
Read depth after trimming	6,424,786
Duplication proportion	0.9298
Proportion reads mapped	0.4523
Exon/intron ratio	0.24545
Unique read proportion	0.03615

**Bidirectionals**
Total # Tfit bidirectionals	52
# Promoter Tfit bidirectionals	36
# Intronic Tfit bidirectionals	31
# Intergenic Tfit bidirectionals	6
Total # dREG bidirectionals	136
# Promoter dREG bidirectionals	23
# Intronic dREG bidirectionals	46
# Intergenic dREG bidirectionals	70
Part of Tfit master merge?	True
Part of dREG master merge?	True