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SRR1991268 Sample ID 2301
| Paper identifier | Sigova2015transcription |
| SRP accession | SRP057598 |
| GEO accession | GSE68197 |
| Samples | SRR1991268 |
| QC score | 5 |
| Replicate | 1 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | cell line |
| Cell type | ESC |
| Clone/Individual | |
| Strain | |
| Genotype | BirA knock-in |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | actinomycin D (1 microM) | -6 hr | 0 hr | 6 hr |
| Pipeline info and software versions | |
| Sample notes | |
| Analysis notes | Sparse due to major poly-A contamination and high duplication; Ran qc redo nextflow |
| Sample QC score | 5 |
| Sample NRO score | 2 |
| Raw read length | 40 |
| Read depth after trimming | 6,424,786 |
| Duplication proportion | 0.9298 |
| Proportion reads mapped | 0.4523 |
| Exon/intron ratio | 0.24545 |
| Unique read proportion | 0.03615 |
| Total # Tfit bidirectionals | 52 |
| # Promoter Tfit bidirectionals | 36 |
| # Intronic Tfit bidirectionals | 31 |
| # Intergenic Tfit bidirectionals | 6 |
| Total # dREG bidirectionals | 136 |
| # Promoter dREG bidirectionals | 23 |
| # Intronic dREG bidirectionals | 46 |
| # Intergenic dREG bidirectionals | 70 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |