DBNascent | Samples

SRR2036963 Sample ID 785

**Data sources**
Paper identifier	Flynn20167skbaf
SRP	SRP058597
Samples	SRR2036963

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	113 Mb
Stranded gene read counts	TXT file	924 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	V6.5
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	7SK antisense oligonucleotide (1 nmole/2 million cells)	-12 hr	0 hr	12 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	dirty data - did fastq-dump manually, force trimmed first 25 bp and bp 65+ prior to running pipeline, ran pipeline on trimmed fastqs (with normal bbduk trimming included), a few samples still sparse due to major adapter contamination

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	41
Read depth after trimming	44,107,188
Duplication proportion	0.46368
Proportion reads mapped	0.7542
Exon/intron ratio	2.96128
Unique read proportion	0.62106

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False