DBNascent | Samples

SRR2036965 Sample ID 787

**Data sources**
Paper identifier	Flynn20167skbaf
SRP	SRP058597
Samples	SRR2036965

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	control
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	17 Mb
Stranded gene read counts	TXT file	899 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	493 Kb
dREG bidirectional regions	BED file	186 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	V6.5
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	control antisense oligonucleotide (1 nmole/2 million cells)	-12 hr	0 hr	12 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	dirty data - did fastq-dump manually, force trimmed first 25 bp and bp 65+ prior to running pipeline, ran pipeline on trimmed fastqs (with normal bbduk trimming included), a few samples still sparse due to major adapter contamination

**Sample metrics**
Sample QC score	5
Sample NRO score	5
Raw read length	41
Read depth after trimming	65,229,033
Duplication proportion	0.96701
Proportion reads mapped	0.7279
Exon/intron ratio	30.7588
Unique read proportion	0.03718

**Bidirectionals**
Total # Tfit bidirectionals	5852
# Promoter Tfit bidirectionals	2533
# Intronic Tfit bidirectionals	1531
# Intergenic Tfit bidirectionals	793
Total # dREG bidirectionals	4322
# Promoter dREG bidirectionals	2587
# Intronic dREG bidirectionals	707
# Intergenic dREG bidirectionals	576
Part of Tfit master merge?	False
Part of dREG master merge?	False