DBNascent | Samples

SRR2129943 Sample ID 2742

**Data sources**
Paper identifier	Yu2015panoramix
SRP accession	SRP061628
GEO accession	GSE71369
Samples	SRR2129943

**Technical details**
QC score	5
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	26 Mb
Stranded gene read counts	TXT file	712 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	tissue
Cell type	ovary
Clone/Individual
Strain
Genotype	nanos promoter Gal4-responsive dsRNA CG9754 knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Library prep not explicitly stated but refers to Huppertz et al 2014 Methods, which is circularization
Analysis notes	dirty data - did fastq-dump manually, force trimmed first 15 bp and bp 45+ prior to running pipeline, ran pipeline on trimmed fastqs (with normal bbduk trimming included); a few samples sparse due to low read depth

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	10
Read depth after trimming	4,770,306
Duplication proportion	0.58093
Proportion reads mapped	0.5989
Exon/intron ratio	1.68793
Unique read proportion	0.25031

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False