DBNascent | Samples

SRR2129946 Sample ID 2745

**Data sources**
Paper identifier	Yu2015panoramix
SRP accession	SRP061628
GEO accession	GSE71369
Samples	SRR2129946

**Technical details**
QC score	5
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	9 Mb
Stranded gene read counts	TXT file	697 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	tissue
Cell type	ovary
Clone/Individual
Strain
Genotype	luciferase knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Library prep not explicitly stated but refers to Huppertz et al 2014 Methods, which is circularization
Analysis notes	dirty data - did fastq-dump manually, force trimmed first 15 bp and bp 45+ prior to running pipeline, ran pipeline on trimmed fastqs (with normal bbduk trimming included); a few samples sparse due to low read depth

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	10
Read depth after trimming	457,270
Duplication proportion	0.45385
Proportion reads mapped	0.699
Exon/intron ratio	1.40404
Unique read proportion	0.08326

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False