DBNascent | Samples

SRR2144182 Sample ID 1781

**Data sources**
Paper identifier	Mahat2016mammalian
SRP accession	SRP062019
GEO accession	GSE71708
Samples	SRR2144182

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	81 Mb
Stranded gene read counts	TXT file	918 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	766 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat shock (42 Celsius)	-12 min	0 min	12 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	35
Read depth after trimming	50,821,238
Duplication proportion	0.60137
Proportion reads mapped	0.587
Exon/intron ratio	3.97749
Unique read proportion	0.49832

**Bidirectionals**
Total # Tfit bidirectionals	24648
# Promoter Tfit bidirectionals	10741
# Intronic Tfit bidirectionals	7932
# Intergenic Tfit bidirectionals	3607
Total # dREG bidirectionals	17548
# Promoter dREG bidirectionals	6979
# Intronic dREG bidirectionals	5582
# Intergenic dREG bidirectionals	3094
Part of Tfit master merge?	True
Part of dREG master merge?	True