DBNascent | Samples

SRR2144189 Sample ID 1788

**Data sources**
Paper identifier	Mahat2016mammalian
SRP accession	SRP062019
GEO accession	GSE71708
Samples	SRR2144189

**Technical details**
QC score	4
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	32 Mb
Stranded gene read counts	TXT file	908 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	506 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain
Genotype	Hsf1 -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat shock (42 Celsius)	-150 s	0 s	150 s

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	9,706,395
Duplication proportion	0.42193
Proportion reads mapped	0.7237
Exon/intron ratio	3.84746
Unique read proportion	0.54234

**Bidirectionals**
Total # Tfit bidirectionals	19394
# Promoter Tfit bidirectionals	7366
# Intronic Tfit bidirectionals	6995
# Intergenic Tfit bidirectionals	2978
Total # dREG bidirectionals	11743
# Promoter dREG bidirectionals	5931
# Intronic dREG bidirectionals	3192
# Intergenic dREG bidirectionals	1540
Part of Tfit master merge?	True
Part of dREG master merge?	True