DBNascent | Samples

SRR2153513 Sample ID 2136

**Data sources**
Paper identifier	Salony2016akt
SRP accession	SRP062229
GEO accession	GSE71898
Samples	SRR2153513

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	44 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	257 Kb
dREG bidirectional regions	BED file	474 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	AKTi-1/2 (20 microM)	-72 hr	0 hr	3 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	50
Read depth after trimming	91,451,663
Duplication proportion	0.92976
Proportion reads mapped	0.6452
Exon/intron ratio	1.67594
Unique read proportion	0.24695

**Bidirectionals**
Total # Tfit bidirectionals	3027
# Promoter Tfit bidirectionals	204
# Intronic Tfit bidirectionals	2135
# Intergenic Tfit bidirectionals	357
Total # dREG bidirectionals	11034
# Promoter dREG bidirectionals	145
# Intronic dREG bidirectionals	8401
# Intergenic dREG bidirectionals	1512
Part of Tfit master merge?	True
Part of dREG master merge?	True