DBNascent | Samples

SRR2153515 Sample ID 2138

**Data sources**
Paper identifier	Salony2016akt
SRP accession	SRP062229
GEO accession	GSE71898
Samples	SRR2153515

**Technical details**
QC score	5
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	15 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	11 Kb
dREG bidirectional regions	BED file	33 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-72 hr	0 hr	3 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	5
Raw read length	50
Read depth after trimming	174,322,983
Duplication proportion	0.99457
Proportion reads mapped	0.7993
Exon/intron ratio	0.37443
Unique read proportion	0.0352

**Bidirectionals**
Total # Tfit bidirectionals	135
# Promoter Tfit bidirectionals	33
# Intronic Tfit bidirectionals	102
# Intergenic Tfit bidirectionals	5
Total # dREG bidirectionals	760
# Promoter dREG bidirectionals	15
# Intronic dREG bidirectionals	594
# Intergenic dREG bidirectionals	84
Part of Tfit master merge?	True
Part of dREG master merge?	True