DBNascent | Samples

SRR2432161 Sample ID 1191

**Data sources**
Paper identifier	Kantidakis2016mutation
SRP accession	SRP063847
GEO accession	GSE73126
Samples	SRR2432161

**Technical details**
QC score	1
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	111 Mb
Stranded gene read counts	TXT file	923 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	950 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain
Genotype	Mll2 fl/fl; tamoxifen-inducible Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	hydroxytamoxifen (1 microM)	-24 hr	0 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	when cells were treated with tamoxifen for 24 h, it resulted in the complete excision of MLL2 exons 3 to 5
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	5
Raw read length	51
Read depth after trimming	42,570,273
Duplication proportion	0.48021
Proportion reads mapped	0.8095
Exon/intron ratio	12.437
Unique read proportion	0.64922

**Bidirectionals**
Total # Tfit bidirectionals	29016
# Promoter Tfit bidirectionals	6899
# Intronic Tfit bidirectionals	12389
# Intergenic Tfit bidirectionals	3734
Total # dREG bidirectionals	21673
# Promoter dREG bidirectionals	4172
# Intronic dREG bidirectionals	8896
# Intergenic dREG bidirectionals	4161
Part of Tfit master merge?	True
Part of dREG master merge?	True