DBNascent | Samples

SRR2518188 Sample ID 2388

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP accession	SRP064201
GEO accession	GSE73441
Samples	SRR2518188

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	36 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	336 Kb
dREG bidirectional regions	BED file	125 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	27,070,240
Duplication proportion	0.7708
Proportion reads mapped	0.7935
Exon/intron ratio	3.3484
Unique read proportion	0.24424

**Bidirectionals**
Total # Tfit bidirectionals	3930
# Promoter Tfit bidirectionals	732
# Intronic Tfit bidirectionals	1661
# Intergenic Tfit bidirectionals	472
Total # dREG bidirectionals	2914
# Promoter dREG bidirectionals	502
# Intronic dREG bidirectionals	988
# Intergenic dREG bidirectionals	1125
Part of Tfit master merge?	False
Part of dREG master merge?	False