DBNascent | Samples

SRR2518194 Sample ID 2394

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP accession	SRP064201
GEO accession	GSE73441
Samples	SRR2518194

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	16 Mb
Stranded gene read counts	TXT file	898 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	232 Kb
dREG bidirectional regions	BED file	68 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	flavopiridol (300 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	21,949,534
Duplication proportion	0.91092
Proportion reads mapped	0.7568
Exon/intron ratio	3.944
Unique read proportion	0.09538

**Bidirectionals**
Total # Tfit bidirectionals	2747
# Promoter Tfit bidirectionals	492
# Intronic Tfit bidirectionals	923
# Intergenic Tfit bidirectionals	163
Total # dREG bidirectionals	1592
# Promoter dREG bidirectionals	269
# Intronic dREG bidirectionals	494
# Intergenic dREG bidirectionals	522
Part of Tfit master merge?	False
Part of dREG master merge?	False