DBNascent | Samples

SRR2518195 Sample ID 2395

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP accession	SRP064201
GEO accession	GSE73441
Samples	SRR2518195

**Technical details**
QC score	4
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	18 Mb
Stranded gene read counts	TXT file	898 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	242 Kb
dREG bidirectional regions	BED file	70 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	flavopiridol (300 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	51
Read depth after trimming	25,169,889
Duplication proportion	0.91004
Proportion reads mapped	0.7468
Exon/intron ratio	3.03656
Unique read proportion	0.09457

**Bidirectionals**
Total # Tfit bidirectionals	2863
# Promoter Tfit bidirectionals	482
# Intronic Tfit bidirectionals	996
# Intergenic Tfit bidirectionals	130
Total # dREG bidirectionals	1637
# Promoter dREG bidirectionals	247
# Intronic dREG bidirectionals	532
# Intergenic dREG bidirectionals	557
Part of Tfit master merge?	False
Part of dREG master merge?	False