DBNascent | Samples

SRR2518196 Sample ID 2395

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP	SRP064201
Samples	SRR2518196

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	control
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	35 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	294 Kb
dREG bidirectional regions	BED file	106 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype	dox-inducible shRNA control (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	doxycycline (2 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	21,783,629
Duplication proportion	0.74141
Proportion reads mapped	0.8087
Exon/intron ratio	3.33391
Unique read proportion	0.27763

**Bidirectionals**
Total # Tfit bidirectionals	3424
# Promoter Tfit bidirectionals	573
# Intronic Tfit bidirectionals	1399
# Intergenic Tfit bidirectionals	503
Total # dREG bidirectionals	2463
# Promoter dREG bidirectionals	425
# Intronic dREG bidirectionals	804
# Intergenic dREG bidirectionals	1014
Part of Tfit master merge?	False
Part of dREG master merge?	False