DBNascent | Samples

SRR2518198 Sample ID 2397

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP	SRP064201
Samples	SRR2518198

**Technical details**
QC score	3
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	control
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	30 Mb
Stranded gene read counts	TXT file	908 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	293 Kb
dREG bidirectional regions	BED file	93 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype	dox-inducible shRNA control (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	doxycycline (2 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	21,379,998
Duplication proportion	0.76986
Proportion reads mapped	0.7793
Exon/intron ratio	3.1283
Unique read proportion	0.24596

**Bidirectionals**
Total # Tfit bidirectionals	3423
# Promoter Tfit bidirectionals	682
# Intronic Tfit bidirectionals	1276
# Intergenic Tfit bidirectionals	439
Total # dREG bidirectionals	2153
# Promoter dREG bidirectionals	430
# Intronic dREG bidirectionals	653
# Intergenic dREG bidirectionals	807
Part of Tfit master merge?	False
Part of dREG master merge?	False