DBNascent | Samples

SRR2518200 Sample ID 2399

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP	SRP064201
Samples	SRR2518200

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	36 Mb
Stranded gene read counts	TXT file	909 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	578 Kb
dREG bidirectional regions	BED file	263 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype	dox-inducible shRNA Phf5a (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	doxycycline (2 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	3
Raw read length	51
Read depth after trimming	19,537,259
Duplication proportion	0.7504
Proportion reads mapped	0.728
Exon/intron ratio	5.62222
Unique read proportion	0.27145

**Bidirectionals**
Total # Tfit bidirectionals	6818
# Promoter Tfit bidirectionals	631
# Intronic Tfit bidirectionals	3351
# Intergenic Tfit bidirectionals	1426
Total # dREG bidirectionals	6119
# Promoter dREG bidirectionals	265
# Intronic dREG bidirectionals	2520
# Intergenic dREG bidirectionals	3044
Part of Tfit master merge?	False
Part of dREG master merge?	False