DBNascent | Samples

SRR2518202 Sample ID 2401

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP	SRP064201
Samples	SRR2518202

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	43 Mb
Stranded gene read counts	TXT file	914 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	500 Kb
dREG bidirectional regions	BED file	175 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype	dox-inducible shRNA Paf1 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	doxycycline (2 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	30,780,845
Duplication proportion	0.7596
Proportion reads mapped	0.7251
Exon/intron ratio	3.28571
Unique read proportion	0.31453

**Bidirectionals**
Total # Tfit bidirectionals	5814
# Promoter Tfit bidirectionals	1550
# Intronic Tfit bidirectionals	2280
# Intergenic Tfit bidirectionals	713
Total # dREG bidirectionals	4050
# Promoter dREG bidirectionals	1063
# Intronic dREG bidirectionals	1343
# Intergenic dREG bidirectionals	1122
Part of Tfit master merge?	False
Part of dREG master merge?	False