DBNascent | Samples

SRR2518203 Sample ID 2402

**Data sources**
Paper identifier	Strikoudis2016regulation
SRP	SRP064201
Samples	SRR2518203

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	38 Mb
Stranded gene read counts	TXT file	912 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	487 Kb
dREG bidirectional regions	BED file	150 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	KH2
Strain	C57BL/6 x 129/Sv
Genotype	dox-inducible shRNA Paf1 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	LIF (1000 U/mL)	None	None	None
treatment	doxycycline (2 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	LIF treatment maintains pluripotency
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	51
Read depth after trimming	28,576,140
Duplication proportion	0.76574
Proportion reads mapped	0.6952
Exon/intron ratio	3.62482
Unique read proportion	0.28871

**Bidirectionals**
Total # Tfit bidirectionals	5674
# Promoter Tfit bidirectionals	1454
# Intronic Tfit bidirectionals	2234
# Intergenic Tfit bidirectionals	603
Total # dREG bidirectionals	3492
# Promoter dREG bidirectionals	954
# Intronic dREG bidirectionals	1127
# Intergenic dREG bidirectionals	910
Part of Tfit master merge?	False
Part of dREG master merge?	False