DBNascent | Samples

SRR2634653 Sample ID 2014

**Data sources**
Paper identifier	Orioli2016human
SRP accession	SRP064802
GEO accession	GSE73929
Samples	SRR2634653

**Technical details**
QC score	4
Replicate	3
Protocol	EU-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	10 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file	51 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	IMR90
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	ethynyl uridine (250 microM)	-5 hr	0 hr	5 hr
treatment	serum starvation	-5 hr	0 hr	5 hr

**Extended notes**
Pipeline info and software versions
Sample notes	neusRNA-seq small tRNA incorporate EU
Analysis notes	Unstranded, some quite sparse due to adapter/other contamination, did not map well, but force trimming did not help

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	101
Read depth after trimming	15,757,711
Duplication proportion	0.90777
Proportion reads mapped	0.3654
Exon/intron ratio	2.98438
Unique read proportion	0.06334

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	1179
# Promoter dREG bidirectionals	218
# Intronic dREG bidirectionals	419
# Intergenic dREG bidirectionals	434
Part of Tfit master merge?	False
Part of dREG master merge?	False