DBNascent | Samples

SRR3051603 Sample ID 406

**Data sources**
Paper identifier	Busslinger2017cohesin
SRP accession	SRP067744
GEO accession	GSE76303
Samples	SRR3051603

**Technical details**
QC score	5
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	19 Mb
Stranded gene read counts	TXT file	894 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	129 Kb
dREG bidirectional regions	BED file	18 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype	Ctcf fl/fl; WapI fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Low read depth and strange looking (almost but not quite single stranded); Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	18
Read depth after trimming	3,554,903
Duplication proportion	0.07686
Proportion reads mapped	0.5481
Exon/intron ratio	2.08182
Unique read proportion	0.84

**Bidirectionals**
Total # Tfit bidirectionals	1561
# Promoter Tfit bidirectionals	702
# Intronic Tfit bidirectionals	389
# Intergenic Tfit bidirectionals	143
Total # dREG bidirectionals	419
# Promoter dREG bidirectionals	109
# Intronic dREG bidirectionals	88
# Intergenic dREG bidirectionals	110
Part of Tfit master merge?	True
Part of dREG master merge?	True