DBNascent | Samples

SRR3051604 Sample ID 407

**Data sources**
Paper identifier	Busslinger2017cohesin
SRP accession	SRP067744
GEO accession	GSE76303
Samples	SRR3051604

**Technical details**
QC score	5
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	15 Mb
Stranded gene read counts	TXT file	892 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	371 Kb
dREG bidirectional regions	BED file	44 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype	Ctcf fl/fl; WapI fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	adeno-Cre virus (1 microL/million cells)	-244 hr	-240 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Low read depth and strange looking (almost but not quite single stranded); Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	18
Read depth after trimming	2,583,942
Duplication proportion	0.19909
Proportion reads mapped	0.5644
Exon/intron ratio	2.69697
Unique read proportion	0.62795

**Bidirectionals**
Total # Tfit bidirectionals	4515
# Promoter Tfit bidirectionals	2324
# Intronic Tfit bidirectionals	1275
# Intergenic Tfit bidirectionals	482
Total # dREG bidirectionals	1033
# Promoter dREG bidirectionals	597
# Intronic dREG bidirectionals	192
# Intergenic dREG bidirectionals	130
Part of Tfit master merge?	True
Part of dREG master merge?	True