DBNascent | Samples

SRR3051605 Sample ID 408

**Data sources**
Paper identifier	Busslinger2017cohesin
SRP accession	SRP067744
GEO accession	GSE76303
Samples	SRR3051605

**Technical details**
QC score	5
Replicate	4
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	20 Mb
Stranded gene read counts	TXT file	894 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	138 Kb
dREG bidirectional regions	BED file	15 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype	Ctcf fl/fl; WapI fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Low read depth and strange looking (almost but not quite single stranded); Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	18
Read depth after trimming	3,820,395
Duplication proportion	0.12561
Proportion reads mapped	0.5475
Exon/intron ratio	1.53333
Unique read proportion	0.77158

**Bidirectionals**
Total # Tfit bidirectionals	1675
# Promoter Tfit bidirectionals	834
# Intronic Tfit bidirectionals	433
# Intergenic Tfit bidirectionals	154
Total # dREG bidirectionals	349
# Promoter dREG bidirectionals	132
# Intronic dREG bidirectionals	69
# Intergenic dREG bidirectionals	69
Part of Tfit master merge?	True
Part of dREG master merge?	True