DBNascent | Samples

SRR3051606 Sample ID 409

**Data sources**
Paper identifier	Busslinger2017cohesin
SRP	SRP067744
Samples	SRR3051606

**Technical details**
QC score	5
Replicate	3
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Timecourse	True
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	11 Mb
Stranded gene read counts	TXT file	888 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	237 Kb
dREG bidirectional regions	BED file	28 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype	Ctcf fl/fl; WapI fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	adeno-Cre virus (1 microL/million cells)	-244 hr	-240 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Low read depth and strange looking (almost but not quite single stranded); Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	4
Raw read length	18
Read depth after trimming	1,447,803
Duplication proportion	0.22124
Proportion reads mapped	0.5782
Exon/intron ratio	7.0
Unique read proportion	0.42317

**Bidirectionals**
Total # Tfit bidirectionals	2888
# Promoter Tfit bidirectionals	1419
# Intronic Tfit bidirectionals	688
# Intergenic Tfit bidirectionals	225
Total # dREG bidirectionals	654
# Promoter dREG bidirectionals	244
# Intronic dREG bidirectionals	127
# Intergenic dREG bidirectionals	137
Part of Tfit master merge?	True
Part of dREG master merge?	True