DBNascent | Samples

SRR3160418 Sample ID 475

**Data sources**
Paper identifier	Compagno2017phosphatidylinositol
SRP accession	SRP069868
GEO accession	GSE77788
Samples	SRR3160418

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	143 Mb
Stranded gene read counts	TXT file	922 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	967 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/mL)	-2 day	0 day	2 day
vehicle	DMSO	-2 day	0 day	2 day
treatment	anti-CD40 (1 microg/mL)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	Library preparation not stated in paper or on GEO "GRO-Seq libraries were prepared as previously described by Core et al., Science 2008"; treatment times unclear
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	51
Read depth after trimming	55,893,066
Duplication proportion	0.51666
Proportion reads mapped	0.8224
Exon/intron ratio	2.45807
Unique read proportion	0.68914

**Bidirectionals**
Total # Tfit bidirectionals	30104
# Promoter Tfit bidirectionals	8422
# Intronic Tfit bidirectionals	12626
# Intergenic Tfit bidirectionals	4648
Total # dREG bidirectionals	22048
# Promoter dREG bidirectionals	4473
# Intronic dREG bidirectionals	9414
# Intergenic dREG bidirectionals	5187
Part of Tfit master merge?	True
Part of dREG master merge?	True