DBNascent | Samples

SRR3317163 Sample ID 1609

**Data sources**
Paper identifier	Liu2013brd4
SRP accession	SRP031885
GEO accession	GSE51633
Samples	SRR3317163

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	83 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	547 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype
Construct	siRNA Brd4

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	siRNA Brd4 #1
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	50
Read depth after trimming	42,402,122
Duplication proportion	0.67868
Proportion reads mapped	0.8213
Exon/intron ratio	2.8691
Unique read proportion	0.39127

**Bidirectionals**
Total # Tfit bidirectionals	18322
# Promoter Tfit bidirectionals	9469
# Intronic Tfit bidirectionals	6876
# Intergenic Tfit bidirectionals	1596
Total # dREG bidirectionals	12699
# Promoter dREG bidirectionals	7170
# Intronic dREG bidirectionals	3882
# Intergenic dREG bidirectionals	1826
Part of Tfit master merge?	True
Part of dREG master merge?	True