DBNascent | Samples

SRR3407137 Sample ID 552

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession	GSE110464
Samples	SRR3407137

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	31 Mb
Stranded gene read counts	TXT file	907 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	615 Kb
dREG bidirectional regions	BED file	208 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/microL)	-25 hr	-1 hr	1 day
treatment	rosiglitazone (10 microM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	30
Read depth after trimming	24,122,094
Duplication proportion	0.68498
Proportion reads mapped	0.5224
Exon/intron ratio	3.63519
Unique read proportion	0.34094

**Bidirectionals**
Total # Tfit bidirectionals	7256
# Promoter Tfit bidirectionals	2372
# Intronic Tfit bidirectionals	2896
# Intergenic Tfit bidirectionals	582
Total # dREG bidirectionals	4831
# Promoter dREG bidirectionals	2393
# Intronic dREG bidirectionals	1202
# Intergenic dREG bidirectionals	511
Part of Tfit master merge?	True
Part of dREG master merge?	True