DBNascent | Samples

SRR3407138 Sample ID 553

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession	GSE110464
Samples	SRR3407138

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	24 Mb
Stranded gene read counts	TXT file	904 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	471 Kb
dREG bidirectional regions	BED file	138 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/microL)	-25 hr	-1 hr	1 day
vehicle	DMSO/ethanol	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	30
Read depth after trimming	18,761,218
Duplication proportion	0.66818
Proportion reads mapped	0.4508
Exon/intron ratio	3.80441
Unique read proportion	0.33204

**Bidirectionals**
Total # Tfit bidirectionals	5554
# Promoter Tfit bidirectionals	1812
# Intronic Tfit bidirectionals	2185
# Intergenic Tfit bidirectionals	415
Total # dREG bidirectionals	3206
# Promoter dREG bidirectionals	1684
# Intronic dREG bidirectionals	769
# Intergenic dREG bidirectionals	262
Part of Tfit master merge?	True
Part of dREG master merge?	True