DBNascent | Samples

SRR3407139 Sample ID 554

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession	GSE110464
Samples	SRR3407139

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	29 Mb
Stranded gene read counts	TXT file	906 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	565 Kb
dREG bidirectional regions	BED file	165 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/microL)	-25 hr	-1 hr	1 day
treatment	rosiglitazone (10 microM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	30
Read depth after trimming	22,076,552
Duplication proportion	0.67986
Proportion reads mapped	0.5229
Exon/intron ratio	3.46154
Unique read proportion	0.33769

**Bidirectionals**
Total # Tfit bidirectionals	6646
# Promoter Tfit bidirectionals	1938
# Intronic Tfit bidirectionals	2715
# Intergenic Tfit bidirectionals	655
Total # dREG bidirectionals	3849
# Promoter dREG bidirectionals	1799
# Intronic dREG bidirectionals	1033
# Intergenic dREG bidirectionals	466
Part of Tfit master merge?	True
Part of dREG master merge?	True