DBNascent | Samples

SRR3407140 Sample ID 555

**Data sources**
Paper identifier	Daniel2018nuclear
SRP accession	SRP073648
GEO accession	GSE110464
Samples	SRR3407140

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	28 Mb
Stranded gene read counts	TXT file	905 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	533 Kb
dREG bidirectional regions	BED file	152 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	bone marrow derived macrophage
Clone/Individual
Strain	C57BL/6
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (20 ng/microL)	-25 hr	-1 hr	1 day
vehicle	DMSO/ethanol	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	30
Read depth after trimming	20,768,534
Duplication proportion	0.6509
Proportion reads mapped	0.4856
Exon/intron ratio	3.5473
Unique read proportion	0.35931

**Bidirectionals**
Total # Tfit bidirectionals	6278
# Promoter Tfit bidirectionals	1950
# Intronic Tfit bidirectionals	2531
# Intergenic Tfit bidirectionals	540
Total # dREG bidirectionals	3544
# Promoter dREG bidirectionals	1747
# Intronic dREG bidirectionals	913
# Intergenic dREG bidirectionals	363
Part of Tfit master merge?	True
Part of dREG master merge?	True