DBNascent | Samples

SRR3500459 Sample ID 898

**Data sources**
Paper identifier	Gibson2016chemical
SRP accession	SRP064970
GEO accession	GSE74142
Samples	SRR3500459

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	83 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	949 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	shRNA luciferase (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	PJ34 (1 microM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	1
Raw read length	43
Read depth after trimming	22,533,256
Duplication proportion	0.3651
Proportion reads mapped	0.8462
Exon/intron ratio	2.79289
Unique read proportion	0.67709

**Bidirectionals**
Total # Tfit bidirectionals	30697
# Promoter Tfit bidirectionals	14588
# Intronic Tfit bidirectionals	11552
# Intergenic Tfit bidirectionals	5031
Total # dREG bidirectionals	21846
# Promoter dREG bidirectionals	9708
# Intronic dREG bidirectionals	8246
# Intergenic dREG bidirectionals	4070
Part of Tfit master merge?	True
Part of dREG master merge?	True