DBNascent | Samples

SRR3535363 Sample ID 2735

**Data sources**
Paper identifier	Yang2017glucocorticoid
SRP accession	SRP075268
GEO accession	GSE81511
Samples	SRR3535363

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	104 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	806 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	ICI 182,780 (100 nM)	-3 hr	0 hr	3 hr
treatment	dexamethasone (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Treatment timing slightly unclear
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	50
Read depth after trimming	37,639,224
Duplication proportion	0.54678
Proportion reads mapped	0.9309
Exon/intron ratio	2.96645
Unique read proportion	0.48956

**Bidirectionals**
Total # Tfit bidirectionals	22032
# Promoter Tfit bidirectionals	9202
# Intronic Tfit bidirectionals	8858
# Intergenic Tfit bidirectionals	4054
Total # dREG bidirectionals	18605
# Promoter dREG bidirectionals	7868
# Intronic dREG bidirectionals	7483
# Intergenic dREG bidirectionals	3500
Part of Tfit master merge?	True
Part of dREG master merge?	True