DBNascent | Samples

SRR3535365 Sample ID 2737

**Data sources**
Paper identifier	Yang2017glucocorticoid
SRP accession	SRP075268
GEO accession	GSE81511
Samples	SRR3535365

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	75 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	454 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-1 hr	0 hr	1 hr
treatment	dexamethasone (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	34,359,854
Duplication proportion	0.65975
Proportion reads mapped	0.9527
Exon/intron ratio	3.48921
Unique read proportion	0.36145

**Bidirectionals**
Total # Tfit bidirectionals	13950
# Promoter Tfit bidirectionals	6395
# Intronic Tfit bidirectionals	5374
# Intergenic Tfit bidirectionals	1669
Total # dREG bidirectionals	10525
# Promoter dREG bidirectionals	5600
# Intronic dREG bidirectionals	3561
# Intergenic dREG bidirectionals	1508
Part of Tfit master merge?	True
Part of dREG master merge?	True