DBNascent | Samples

SRR3541138 Sample ID 2433

**Data sources**
Paper identifier	Tan2018dismissal
SRP accession	SRP250948
GEO accession	GSE73957
Samples	SRR3541138

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	118 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	625 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	Kdm2a HA-tag (stable)
Construct	siRNA Kdm2a 5'UTR

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	50
Read depth after trimming	51,553,380
Duplication proportion	0.60807
Proportion reads mapped	0.8484
Exon/intron ratio	2.47782
Unique read proportion	0.51648

**Bidirectionals**
Total # Tfit bidirectionals	19228
# Promoter Tfit bidirectionals	6770
# Intronic Tfit bidirectionals	8244
# Intergenic Tfit bidirectionals	3201
Total # dREG bidirectionals	14486
# Promoter dREG bidirectionals	6308
# Intronic dREG bidirectionals	5748
# Intergenic dREG bidirectionals	2325
Part of Tfit master merge?	True
Part of dREG master merge?	True