DBNascent | Samples

SRR3619367 Sample ID 2529

**Data sources**
Paper identifier	Vian2018energetics
SRP accession	SRP075985
GEO accession	GSE82144
Samples	SRR3619367

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	333 Mb
Stranded gene read counts	TXT file	928 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Myc fl/fl; tamoxifen-inducible Cre/+
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD180 (500 ng/mL)	-3 hr	0 hr	3 hr
treatment	hydroxytamoxifen	None	None	None
treatment	interleukin-4 (2.5 ng/mL)	-3 hr	0 hr	3 hr
treatment	lipopolysaccharide (50 microg/mL)	-3 hr	0 hr	3 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Mice treated with 1mg/mouse tamoxifen at 4, 3 and 1 day before sacrifice; Poor description of cells and treatment methods
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	196,649,519
Duplication proportion	0.65764
Proportion reads mapped	0.795
Exon/intron ratio	4.07568
Unique read proportion	0.56229

**Bidirectionals**
Total # Tfit bidirectionals	24284
# Promoter Tfit bidirectionals	11178
# Intronic Tfit bidirectionals	6573
# Intergenic Tfit bidirectionals	4630
Total # dREG bidirectionals	29771
# Promoter dREG bidirectionals	5438
# Intronic dREG bidirectionals	12260
# Intergenic dREG bidirectionals	9396
Part of Tfit master merge?	False
Part of dREG master merge?	False