DBNascent | Samples

SRR3619368 Sample ID 2530

**Data sources**
Paper identifier	Vian2018energetics
SRP accession	SRP075985
GEO accession	GSE82144
Samples	SRR3619368

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	324 Mb
Stranded gene read counts	TXT file	928 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Myc fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	anti-CD180 (500 ng/mL)	-3 hr	0 hr	3 hr
treatment	interleukin-4 (2.5 ng/mL)	-3 hr	0 hr	3 hr
treatment	lipopolysaccharide (50 microg/mL)	-3 hr	0 hr	3 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Poor description of treatment methods
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	211,566,039
Duplication proportion	0.69495
Proportion reads mapped	0.7961
Exon/intron ratio	4.06885
Unique read proportion	0.51838

**Bidirectionals**
Total # Tfit bidirectionals	22383
# Promoter Tfit bidirectionals	10571
# Intronic Tfit bidirectionals	6288
# Intergenic Tfit bidirectionals	3828
Total # dREG bidirectionals	28015
# Promoter dREG bidirectionals	5187
# Intronic dREG bidirectionals	11752
# Intergenic dREG bidirectionals	8578
Part of Tfit master merge?	False
Part of dREG master merge?	False