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SRR3619368 Sample ID 2530
| Paper identifier | Vian2018energetics |
| SRP accession | SRP075985 |
| GEO accession | GSE82144 |
| Samples | SRR3619368 |
| QC score | 3 |
| Replicate | 1 |
| Protocol | GRO-seq |
| Library prep | circularization |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | forward |
| Reverse complemented? | False |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 324 Mb |
| Stranded gene read counts | TXT file | 928 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 2 Mb |
| dREG bidirectional regions | BED file | 1 Mb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | primary cell |
| Cell type | splenic B cell |
| Clone/Individual | |
| Strain | |
| Genotype | Myc fl/fl |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | anti-CD180 (500 ng/mL) | -3 hr | 0 hr | 3 hr |
| treatment | interleukin-4 (2.5 ng/mL) | -3 hr | 0 hr | 3 hr |
| treatment | lipopolysaccharide (50 microg/mL) | -3 hr | 0 hr | 3 hr |
| Pipeline info and software versions | |
| Sample notes | Poor description of treatment methods |
| Analysis notes |
| Sample QC score | 3 |
| Sample NRO score | 2 |
| Raw read length | 50 |
| Read depth after trimming | 211,566,039 |
| Duplication proportion | 0.69495 |
| Proportion reads mapped | 0.7961 |
| Exon/intron ratio | 4.06885 |
| Unique read proportion | 0.51838 |
| Total # Tfit bidirectionals | 22383 |
| # Promoter Tfit bidirectionals | 10571 |
| # Intronic Tfit bidirectionals | 6288 |
| # Intergenic Tfit bidirectionals | 3828 |
| Total # dREG bidirectionals | 28015 |
| # Promoter dREG bidirectionals | 5187 |
| # Intronic dREG bidirectionals | 11752 |
| # Intergenic dREG bidirectionals | 8578 |
| Part of Tfit master merge? | False |
| Part of dREG master merge? | False |