DBNascent | Samples

SRR3657420 Sample ID 411

**Data sources**
Paper identifier	Busslinger2017cohesin
SRP accession	SRP067744
GEO accession	GSE76303
Samples	SRR3657420

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	61 Mb
Stranded gene read counts	TXT file	919 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	5 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain	C57BL/6
Genotype	Ctcf fl/fl; WapI fl/fl
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-1650 min	-1440 min	210 min
treatment	adeno-Cre virus (1 microL/million cells)	-268 hr	-264 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	18
Read depth after trimming	18,255,221
Duplication proportion	0.4818
Proportion reads mapped	0.8946
Exon/intron ratio	2.80932
Unique read proportion	0.59694

**Bidirectionals**
Total # Tfit bidirectionals	60511
# Promoter Tfit bidirectionals	10594
# Intronic Tfit bidirectionals	25065
# Intergenic Tfit bidirectionals	21828
Total # dREG bidirectionals	41818
# Promoter dREG bidirectionals	9626
# Intronic dREG bidirectionals	17399
# Intergenic dREG bidirectionals	13626
Part of Tfit master merge?	True
Part of dREG master merge?	True