DBNascent | Samples

SRR3996488 Sample ID 641

**Data sources**
Paper identifier	Duarte2016transcription
SRP accession	SRP069335
GEO accession	GSE77607
Samples	SRR3996488

**Technical details**
QC score	3
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	59 Mb
Stranded gene read counts	TXT file	723 Kb
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	D. melanogaster
Sample type	cell line
Cell type	S2
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	dsRNA LacZ (10 microg/mL)	-5 day	0 day	5 day
treatment	heat shock (initially 48 Celsius)	-20 min	0 min	20 min

**Extended notes**
Pipeline info and software versions
Sample notes	Separate control for M1BP RNAi expt; Heat shock was media added at 48C but incubated at 37C for treatment time
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	76
Read depth after trimming	22,826,278
Duplication proportion	0.70216
Proportion reads mapped	0.7532
Exon/intron ratio	2.39365
Unique read proportion	0.34032

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False