DBNascent | Samples

SRR4012429 Sample ID 581

**Data sources**
Paper identifier	Danko2018dynamic
SRP accession	SRP081110
GEO accession	GSE85337
Samples	SRR4012429

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	161 Mb
Stranded gene read counts	TXT file	926 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	CD4+ T cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	ethanol	-30 min	0 min	30 min
vehicle	DMSO	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	References Kwak2013precise for library prep, no specifics in paper or GEO
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	75
Read depth after trimming	83,156,952
Duplication proportion	0.68335
Proportion reads mapped	0.8875
Exon/intron ratio	4.21663
Unique read proportion	0.66397

**Bidirectionals**
Total # Tfit bidirectionals	27235
# Promoter Tfit bidirectionals	9847
# Intronic Tfit bidirectionals	9784
# Intergenic Tfit bidirectionals	5406
Total # dREG bidirectionals	25761
# Promoter dREG bidirectionals	4734
# Intronic dREG bidirectionals	11485
# Intergenic dREG bidirectionals	6361
Part of Tfit master merge?	True
Part of dREG master merge?	True