DBNascent | Samples

SRR4041371 Sample ID 681

**Data sources**
Paper identifier	Engreitz2016local
SRP accession	SRP082334
GEO accession	GSE85798
Samples	SRR4041371

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	92 Mb
Stranded gene read counts	TXT file	916 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	ESC
Clone/Individual
Strain	129/SV-Jae x castaneus
Genotype	linc1319 +2kb instronic pAS insertion (+/-)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Heterozygous mutations to determine cis/trans effects; Library prep is unclear, but citations indicate ligation
Analysis notes	In db as Engreitz2016long until 2/8/21 - all older pipeline reports and out/err scripts reflect that; Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	3
Raw read length	30
Read depth after trimming	19,995,331
Duplication proportion	0.28527
Proportion reads mapped	0.7654
Exon/intron ratio	5.49156
Unique read proportion	0.52651

**Bidirectionals**
Total # Tfit bidirectionals	41025
# Promoter Tfit bidirectionals	14508
# Intronic Tfit bidirectionals	12017
# Intergenic Tfit bidirectionals	11082
Total # dREG bidirectionals	23737
# Promoter dREG bidirectionals	7902
# Intronic dREG bidirectionals	6800
# Intergenic dREG bidirectionals	6407
Part of Tfit master merge?	True
Part of dREG master merge?	True