DBNascent | Samples

SRR4305542 Sample ID 910

**Data sources**
Paper identifier	Godfrey2017mll
SRP accession	SRP082679
GEO accession	GSE85988
Samples	SRR4305542

**Technical details**
QC score	1
Replicate	3
Protocol	TT-seq
Library prep	random primed
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	202 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	938 Kb
dREG bidirectional regions	BED file	371 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	SEM
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	pinometostat (2 microM)	-7 day	0 day	7 day

**Extended notes**
Pipeline info and software versions
Sample notes	4sU-seq
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	3
Raw read length	35
Read depth after trimming	39,181,962
Duplication proportion	0.32407
Proportion reads mapped	0.9537
Exon/intron ratio	6.8193
Unique read proportion	0.56119

**Bidirectionals**
Total # Tfit bidirectionals	10709
# Promoter Tfit bidirectionals	5110
# Intronic Tfit bidirectionals	4171
# Intergenic Tfit bidirectionals	586
Total # dREG bidirectionals	8585
# Promoter dREG bidirectionals	4114
# Intronic dREG bidirectionals	3112
# Intergenic dREG bidirectionals	994
Part of Tfit master merge?	True
Part of dREG master merge?	True