DBNascent | Samples

SRR4842300 Sample ID 2623

**Data sources**
Paper identifier	Wang2017cell
SRP accession	SRP092486
GEO accession	GSE89464
Samples	SRR4842300

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	182 Mb
Stranded gene read counts	TXT file	931 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Mentions DNA-seq but no indication of available dataset; arbitrarily called sorting time 1 hr
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	190,000,092
Duplication proportion	0.81373
Proportion reads mapped	0.765
Exon/intron ratio	2.60642
Unique read proportion	0.49203

**Bidirectionals**
Total # Tfit bidirectionals	26799
# Promoter Tfit bidirectionals	9819
# Intronic Tfit bidirectionals	8626
# Intergenic Tfit bidirectionals	6337
Total # dREG bidirectionals	31520
# Promoter dREG bidirectionals	6429
# Intronic dREG bidirectionals	13969
# Intergenic dREG bidirectionals	8633
Part of Tfit master merge?	True
Part of dREG master merge?	True