DBNascent | Samples

SRR4842301 Sample ID 2624

**Data sources**
Paper identifier	Wang2017cell
SRP accession	SRP092486
GEO accession	GSE89464
Samples	SRR4842301

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	144 Mb
Stranded gene read counts	TXT file	931 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Mentions DNA-seq but no indication of available dataset; arbitrarily called sorting time 1 hr
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	50
Read depth after trimming	197,669,475
Duplication proportion	0.82985
Proportion reads mapped	0.7871
Exon/intron ratio	3.49435
Unique read proportion	0.47046

**Bidirectionals**
Total # Tfit bidirectionals	23779
# Promoter Tfit bidirectionals	9251
# Intronic Tfit bidirectionals	7960
# Intergenic Tfit bidirectionals	5082
Total # dREG bidirectionals	28149
# Promoter dREG bidirectionals	7042
# Intronic dREG bidirectionals	12018
# Intergenic dREG bidirectionals	7198
Part of Tfit master merge?	True
Part of dREG master merge?	True