DBNascent | Samples

SRR4842302 Sample ID 2624

**Data sources**
Paper identifier	Wang2017cell
SRP	SRP092486
Samples	SRR4842302

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Timecourse	False
Control/Experimental	experimental
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	113 Mb
Stranded gene read counts	TXT file	928 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Fucci-EGFP+ (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting EGFP+	-210 min	-150 min	1 hr
treatment	cell sorting EGFP-	-60 min	0 min	1 hr
treatment	nocodazole (100 ng/mL)	-570 min	-210 min	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Sorted for early G1; arbitrarily called sorting time 1 hr; mentions DNA-seq but no indication of available dataset
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	185,457,316
Duplication proportion	0.85364
Proportion reads mapped	0.7473
Exon/intron ratio	2.19055
Unique read proportion	0.31096

**Bidirectionals**
Total # Tfit bidirectionals	29007
# Promoter Tfit bidirectionals	9095
# Intronic Tfit bidirectionals	10833
# Intergenic Tfit bidirectionals	6667
Total # dREG bidirectionals	29941
# Promoter dREG bidirectionals	6750
# Intronic dREG bidirectionals	13327
# Intergenic dREG bidirectionals	7857
Part of Tfit master merge?	True
Part of dREG master merge?	True