DBNascent | Samples

SRR4842303 Sample ID 2626

**Data sources**
Paper identifier	Wang2017cell
SRP accession	SRP092486
GEO accession	GSE89464
Samples	SRR4842303

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	109 Mb
Stranded gene read counts	TXT file	929 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	910 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	splenic B cell
Clone/Individual
Strain
Genotype	Fucci-EGFP+ (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	cell sorting EGFP+	-210 min	-150 min	1 hr
treatment	cell sorting EGFP-	-60 min	0 min	1 hr
treatment	nocodazole (100 ng/mL)	-570 min	-210 min	6 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Sorted for early G1; arbitrarily called sorting time 1 hr; mentions DNA-seq but no indication of available dataset
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	50
Read depth after trimming	160,487,189
Duplication proportion	0.84305
Proportion reads mapped	0.7987
Exon/intron ratio	3.12564
Unique read proportion	0.44217

**Bidirectionals**
Total # Tfit bidirectionals	19292
# Promoter Tfit bidirectionals	7011
# Intronic Tfit bidirectionals	7285
# Intergenic Tfit bidirectionals	3249
Total # dREG bidirectionals	20881
# Promoter dREG bidirectionals	5421
# Intronic dREG bidirectionals	9079
# Intergenic dREG bidirectionals	4586
Part of Tfit master merge?	True
Part of dREG master merge?	True