DBNascent | Samples

SRR497911 Sample ID 919

**Data sources**
Paper identifier	Hah2011rapid
SRP accession	SRP013239
GEO accession	GSE27463
Samples	SRR497911

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	33 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	388 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-10 min	0 min	10 min

**Extended notes**
Pipeline info and software versions
Sample notes	Also in SRM497911
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	2
Raw read length	44
Read depth after trimming	7,266,821
Duplication proportion	0.38653
Proportion reads mapped	0.7969
Exon/intron ratio	3.03431
Unique read proportion	0.57967

**Bidirectionals**
Total # Tfit bidirectionals	16278
# Promoter Tfit bidirectionals	5222
# Intronic Tfit bidirectionals	7541
# Intergenic Tfit bidirectionals	2599
Total # dREG bidirectionals	9035
# Promoter dREG bidirectionals	5126
# Intronic dREG bidirectionals	2901
# Intergenic dREG bidirectionals	1249
Part of Tfit master merge?	True
Part of dREG master merge?	True